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AHD ELISA Kit

Product Name:

AHD ELISA Kit

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Description:

The AHD ELISA kit quantitatively detects the presence of AHD in meat, seafood, and honey samples.

Shelf Life:

12 months

Detection Principle:

This test kit is based on the solid phase enzyme-linked immunosorbent assay (ELISA) for the detection of AHD. An unknown amount of AHD present in the sample and the fixed amount of AHD antigens pre-coated on the wells of microtiter plate/strips compete for the anti-AHD antibodies, which in turn is detected with enzyme conjugate. After incubation the wells are washed and the bound enzyme is visualised by adding TMB solution. Any coloured product is measured at 450 nm after adding stop solution. The absorbance value of the developed colour is inversely proportional to the amount of the AHD in the sample. The quantity of AHD in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Introduction:

Nitrofurantoin (AHD) is one of the nitrofurans, which are synthetic broad-spectrum antibiotics. Nitrofurans have been banned from use in treating animals used for food production, because of the possibility of increased cancer risk if people are exposed to them over a long period of time.

Kit Contents:

1. 1 x Pre-coated microtiter plate (12 x 8 microwells).
2. 1 x Enzyme conjugate solution (12 ml).
3. 1 x AHD Antibody solution (7 ml).
4. 1 x Wash buffer concentrate (20×, 40 ml).
5. 1 x Extraction solution concentrate (2×, 50 ml).
6. 6 x AHD standards (1.0 ml/each) (0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb, 4.05 ppb).
7. 1 x High Concentration of the standard (1.0 ml, 100 ppb)..
8. 1 x Derivative Reagent (10 ml).
9. 1 x TMB substrate solution (14 ml).
10. 1 x Stop solution (14 ml).
11. 1 x Package insert.
12. 1 x Microplate sealer.

Packaging:

1 x 96 well Plate

Storage Conditions:

Store at 2-8°C.

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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