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Test kit for Potential Anti Oxidant (PAO)

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Application:

Human and animal serum samples, foods and beverage samples.

Assay Range:

21.9 - 4378 µmol/L (cupric ion reducing power)

Detection Method:

Colormetric assay(detection: 480 - 492 nm)

Detection Principle:

In the PAO assay kit, an easy and convenient method to measure antioxidant capacity is provided. Utilizing the reduction of cupric ion (Cu⁺+ to Cu⁺), antioxidant capacity of samples can be detected in 5 minutes. Samples are mixed with Cu⁺+ Solution. Cu⁺+ are reduced by antioxidants to form Cu⁺. Reduced Cu⁺ react with Chromatic Solution (Bathocuproine) , and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu⁺ formed. PAO can detect not only hydrophilic antioxidants such as Vitamin C, glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. Applicable for assessment of total antioxidants of serum, foods and beverage samples.

How To Use:

1) Prepare 6 levels of standards by diluting 2mM uric acid.
2) Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 µL of Sample Diluent, and add 10 µL of standards or diluted samples.
3) Pour 200 µL of mixture to Micro titer plate. Use 200 µL of Sample Diluent for blank well.
4) Read absorbance at 490 nm (as READ1).
5) Add 50 µL of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
6) Add 50 µL of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
7) Please draw standard curves by plotting the difference of absorbance readings (READ2 - READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (µmol/L).
1mM of uric acid = 2189 µmol/L (copper reducing power)

Introduction:

Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. Recently, antioxidants as functional foods which scavenge ROS attract a great deal of attention.

Kit Contents:

1. Standard (Uric acid powder): 1 vial
2. Sample diluent: 1 bottle
3. Cu⁺⁺ solution: 1 bottle
4. Stop solution: 1 bottle
5. Micro titer plate: 1 plate (96 wells)

Packaging:

96 wells

Storage Conditions:

Room temperature (10 - 25°C)

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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