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Soy ELISA Kit

Product Name:

Soy ELISA Kit

Price:

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Description:

Soy (also called Glycine max) and more commonly known as soybean is a member of the legumes that are native to East Asia. These beans are rich in dietary minerals, B vitamins and phytic acid. Soy vegetable oil is often used in many industrial and food applications. The fraction of proteins in soy beans is relatively high (approx. 40%). It is these proteins which lead to allergic reactions (for example: Glycinin, Gly m1, Gly m4 and Kunitz-TrypsinInhibitor). Allergies to soy are common and foods containing these proteins are often listed alongside other allergens such as egg, milk, tree nuts, peanuts and shellfish. Majority of problems tend to be found in younger children and the initial diagnosis of soy allergy tends to be based on the symptoms that have been previously reported by parents, as a result blood test or skin test for the allergy. For an individual that is allergic to soy any hidden soy allergens that are present in food can result in critical problems, even a small amount can lead to allergic reactions and in severe cases cause anaphylactic shock.

Shelf Life:

12 months

Detection Limit:

16.2 ppb

Detection Method:

ELISA

Incubation Time:

60 minutes

Intended Use:

Soy ELISA kit is intended for measuring quantitative levels of soybean trypsin inhibitors (STI) present in soy residues in foods (such as chocolate, ice cream, cookies, cereals, sausage and instant soups). This assay has a minimum analytical sensitivity limit of 16.0 ppb.

Kit Contents:

1. Microtiter Plate: Coated with anti STI antibodies. 2. STI Standards 1-5: Concentration 0, 40, 100, 1000 ppb. 3. Conjugate (Anti-STI-Peroxidase). 4. Substrate Solution (TMB). 5. Stop Solution (0.5 M H₂SO₄). 6. Extraction and sample dilution buffer (Tris)(10x). 7. Washing Solution (PBS + Tween 20)(10x Concentrate). 8. Instruction Manual.

Packaging:

96 well ELISA microplate

Precision:

6-8%

Range of Quantitation:

40.0 ppb

Recovery Rate:

Cookies (106%), Cereals (100%), Sausage (96%), Instant soup (90%), Ice cream, Chocolate (77%).

Sample:

Food (Instant Soups, Chocolate, Sausage, Ice Cream, Cereals, Cookies)

Sample Size:

100 µL

Sensitivity:

The minimum detection sensitivity level of soybean trypsin inhibitors (STI) using current soy ELISA kit was 16.0 ppb. The standard range for this assay is 40.0-1,000.0 ppb.

Storage Conditions:

All reagents supplied need to be stored at 2-8°C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Substrate:

TMB 450 nm

Tags:

Food Analytic / Safety

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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