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ROS Detection Assay Kit (DCFDA / H₂DCFDA)

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Description:

ROS Detection Assay Kit is a highly sensitive and safe assay to measure Reactive Oxygen Species. ROS include a number of reactive molecules and free radicals, derived from molecular oxygen, that damage DNA and RNA and oxidize proteins and lipids (lipid peroxidation). The most common ROS include superoxide anion (O₂•-), hydrogen peroxide (H₂O₂), hydroxyl radical (HO•) and singlet oxygen (O₂), all of which are more reactive than oxygen (O₂) itself. The molecules are produced during the electron transport of mitochondrial aerobic respiration or by oxidoreductase enzymes and metal catalysed oxidation. It uses the cell permeant reagent Dichlorodihydrofluorescein-diacetate (H₂DCFDA), a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, the acetyl groups on H₂DCFDA are cleaved by intracellular esterase to yield the non-fluorescent compound which is rapidly oxidized to highly fluorescent 2’,7’-Dichlorodihydrofluorescein by ROS. The fluorescence intensity is proportional to the ROS levels within the cell cytosol.

Application:

Measurement of intracellular levels of ROS.

Shelf Life:

24 months from the date of purchase, if it is properly stored.

Advantages:

1. Based on unique fluorescent probe H₂DCFDA.
2. Fast & Easy Protocol.
3. Safe: non-Radioactive, no special handling needed.
4. High-Throughput.
5. Accurate & Reproducible results.

Kit Contents:

1. 5 mg, Fluorescent Substrate (H₂DCFDA). 2. 50 mL, 10X Assay Buffer. 3. 500 μL, Positive control (H₂O₂, 8.8M). 4. 1 mL, DMSO.

Packaging:

5×96 assays

Storage Conditions:

4 °C upon receipt.

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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ROS Detection Assay Kit (DCFDA / H2DCFDA)

ROS Detection Assay Kit (DCFDA / H₂DCFDA)