Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form at the bottom. We will get back to you as soon as possible.

ROS Detection Assay Kit

Product Name:

Price:

Inquiry

Description:

The ROS Detection Assay kit (Green Fluorescence) is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2’, 7’-Dichlorodihydrofluorescein diacetate (DCF-DA).

Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidized by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.

Application:

1. Measure ROS production in live cells
2. Ideal for fluorescence microscopy, microplate titration and cytometry
3. Compatible with adherent cells as well as suspension cells

Shelf Life:

12 months

Kit Contents:

1. 25mM DFC-DA in DMF (35µL) - 10X ROS Buffer (5 mL)
2. Tert-Butyl Hydrogen Peroxide (TBHP) 25 mM (50 µL)

Packaging:

300 assays in 96-well plate

Storage Conditions:

Upon reception, store the DFC-DA tube (avoid multiple freeze/thawing cycles) and the 10X ROS Buffer at -20°C. Store the TBHP tube at 4°C.

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

Online Inquiry

Alfa Chemistry is a leading supplier of world-renowned test kits. Our high-quality products and customized services are trustworthy and support you in your innovative discoveries.

Company

Tel:
Fax:
Email: