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Reactive Oxygen Species (ROS) Fluorometric Assay Kit

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Reactive Oxygen Species (ROS) Fluorometric Assay Kit

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Application:

This kit can be used to measure Reactive Oxygen Species (ROS) in tissue and cell samples.

Shelf Life:

6 months

Assay Type:

Cell-based (quantitative)

Detection Method:

Fluorimetric method

Detection Principle:

DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.

Introduction:

Reactive oxygen species (ROS) are active chemical substances produced in the metabolic process of the body, including oxygen free radicals, hydrogen peroxide and its downstream products, such as peroxides and hydroxides. ROS are both necessary and harmful to organisms, and are involved in cell growth, proliferation, development and differentiation, aging and apoptosis, as well as many physiological and pathological processes. Excessive ROS will lead to oxidative stress and oxidative damage of cells, further promoting the occurrence and development of many diseases, such as cancer, cardiovascular disease and diabetes.

Other Instruments Required:

Fluorescence Microplate reader, Fluorescence Microscope, Flow Cytometry

Packaging:

96 Tests

Sample:

Fresh tissue,cultured cells

Storage Conditions:

Store at -20 °C avoid freeze/thaw cycles

Testing Time:

60 minutes

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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