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Reactive Oxygen Species (ROS) Detection Assay Kit

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Description:

Constant generation of low levels of reactive oxygen species (ROS) and free radicals is a basic feature of all living cells. Low levels of ROS play an essential role in signaling pathways, whereas increased under oxidative stress, ROS activity result in damage to nucleic acids, proteins and membrane lipids. Accumulation of ROS during oxidative stress is also associated with aging, apoptosis or necrosis, and is implicated in pathological conditions such as; vascular diseases, diabetes, renal ischemia, arteriosclerosis, pulmonary disorders, inflammatory diseases, and cancer. Cellular activity of ROS is offset by antioxidants, numerous repair systems, and replacement of damaged DNA. Probes for measuring intracellular ROS levels provide important tools to study oxidative stress inducers and effects of antioxidant therapies. BioVision’s ROS Detection Assay Kit is designed for detection of hydroxyl, peroxyl, or other reactive oxygen species in live cells. We utilize H₂DCFDA, a unique cell-permeable fluorogenic probe, compatible with phenol red, FBS and BSA to detect reactive oxygen species in live cells. Upon the cell entry, H₂DCFDA is modified by cellular esterases to form a non-fluorescent H₂DCF. Oxidation of H₂DCF by intracellular ROS yields highly a fluorescent product that can be detected by FACS, microplate reader, or fluorescence microscope (Ex/Em 495/529 nm). The fluorescence intensity is proportional to the ROS levels. Our kit provides a simple and specific assay for the real-time measurement of global levels of ROS in living cells. We include sufficient reagents to perform 100 assays and a common ROS inducer as a control for measurement of ROS levels or antioxidant activity with high sensitivity, specificity and accuracy

Application:

1. Measurement of intracellular levels of ROS
2. Screening compounds with a stress inducing and/or antioxidant function

Shelf Life:

12 months

Advantages:

1. Simple & Rapid Protocol. 2. Convenient: Non-Radioactive, no special handling or disposal required. 3. High-Throughput. 4. Accurate: reproducible results with low intra & inter assay variability. 5. Measurement of intracellular levels of ROS. 6. Screening compounds with a stress inducing and/or antioxidant function.

Intended Use:

Highly Sensitive Assay that measures Reactive Oxygen Species

Kit Contents:

1. ROS Assay Buffer, 65 ml, WM/NM.
2. ROS Label (1000X), 25 µl, Green.
3. ROS Inducer (250X), 20 µl, Yellow.

Packaging:

250 assays

Storage Conditions:

Store at -20 °C avoid freeze/thaw cycles

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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