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Reactive Oxygen Species Colorimetric Assay Kit

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Shelf Life:

12 months

Assay Type:

Biochemical kit

Detection Method:

Fluorescence method

Introduction:

Reactive oxygen species (ROS) including the super oxygen free radical, hydrogen peroxide and its downstream product (peroxide and hydroxide) etc. ROS is involved in cell growth, proliferation, differentiation of development, aging and apoptosis as well as many physiological and disease process. The DCFH-DA (2, 7-dichlorofuorescin diacetate) is the most commonly used and most sensitive intracellular reactive oxygen detection probe. DCFH-DA has no fluorescence, it is hydrolyzed into DCFH (dichlorofluorescin) by ester hydrolysis enzyme after entering cells. DCFH can be oxidized to strong green fluorescence material DCF (dichlorofluorescein) that cannot pass through cell membrane when active oxygen exists. Its fluorescence has the largest wave crest in excitation wavelength of 502 nm and emission wavelength of maximum peak around 530 nm, the intensity is proportional to the level of reactive oxygen species in cells. This ROS detection system has low background, high sensitivity, good repeatability and simple operation.

Other Instruments Required:

Fluorescence Microplate reader, Fluorescence Spectrophotometry, Flow Cytometry

Storage Conditions:

Store at -20 °C
avoid freeze/thaw cycles

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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