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Rat reactive oxygen species( ROS) ELISA Kit

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Shelf Life:

6 months

Advantages:

1. Based on unique fluorescent probe H₂DCFDA.
2. Fast & Easy Protocol.
3. Safe: non-Radioactive, no special handling needed.
4. High-Throughput.
5. Accurate & Reproducible results.

Detection Principle:

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to ROS. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for ROS is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ROS and HRP conjugated ROS antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ROS. You can calculate the concentration of ROS in the samples by comparing the OD of the samples to the standard curve

Intended Use:

Our Rat reactive oxygen species( ROS) ELISA kit is to assay ROS levels in Rat serum, plasma, culture media or any biological fluid.

Storage Conditions:

Storage: 2-8°C.

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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