Description:
Measure NO in various samples by NO2-/NO3-determination
MyBioSource's OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme. Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 uM (in 50 uL sample volume).
Introduction: Reactive nitrogen intermediates (RNI) and reactive oxygen intermediates (ROI) work together to damage cells and have been implicated in the pathogenesis of several disease states. RNI are a family of molecules derived from nitric oxide (NO) and superoxide anion (O2-), produced via nitric oxide synthase (NOS) and NADPH oxidase, respectively. Nitric oxide is an established mediator in vascular diseases, diabetes, renal ischemia, atherosclerosis, inflammatory diseases, and cancer. However, because of its extremely short half life, direct quantitation of NO production remains challenging and unsuitable for most detection systems. Therefore, the common method for measurement of total NO has become the sum of its final oxidized products, nitrite (NO2-) and nitrate (NO3-) (Figure 1).
MyBioSource's OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, fluorometric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme (Figure 2). Next, total nitrite is detected with the fluorescent probe DAN (2,3-diaminonaphthalene). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 500 nM (in 50 uL sample volume)
Sample:
erum, plasma, urine, saliva, lysates, and media