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OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Colorimetric)

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OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Colorimetric)

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Shelf Life:

12 months

Introduction:

Reactive nitrogen intermediates (RNI) and reactive oxygen intermediates (ROI) work together to damage cells and have been implicated in the pathogenesis of several disease states. RNI are a family of molecules derived from nitric oxide (NO) and superoxide anion (O2-), produced via nitric oxide synthase (NOS) and NADPH oxidase, respectively. Nitric oxide is an established mediator in vascular diseases, diabetes, renal ischemia, atherosclerosis, inflammatory diseases, and cancer. However, because of its extremely short half life, direct quantitation of NO production remains challenging and unsuitable for most detection systems. Therefore, the common method for measurement of total NO has become the sum of its final oxidized products, nitrite (NO₂-) and nitrate (NO₃-) (Figure 1). OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO₂-/NO₃- determination. First, the nitrate (NO₃-) in the sample is converted to nitrite (NO₂-) by nitrate reductase enzyme (Figure 2). Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 muM (in 50 muL sample volume)

Packaging:

100 assays

Storage Conditions:

Store at -20°C.
avoid freeze/thaw cycles

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OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Colorimetric)

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OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate)

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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