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Nitrofuran (SEM) ELISA Test Kit

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Shelf Life:

12 months

Cross-reaction Rate:

SEM： 100%
AMOZ： <0.1%
AOZ： <0.1%
AHD： <0.1%

Detection Limit:

Tissue, egg, honey: 0.1 ppb

Detection Principle:

This test kit is based on the competitive enzyme immunoassay for the detection of Nitrofuran (SEM) in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Nitrofuran (SEM) in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the SEM in it. This value is compared to the standard curve and the SEM concentration is subsequently obtained.

Kit Contents:

1. Micro-well strips, 12 strips with 8 removable wells each.
2. 6×standard solution (1 mL each): 0 ppb, 0.04 ppb, 0.12 ppb, 0.36 ppb, 1.08 ppb, 3.24 ppb.
3. Enzyme conjugate, 7 mL, red cap.
4. Antibody working solution, 7 mL, blue cap.
5. Substrate A, 7 mL, white cap.
6. Substrate B, 7 mL, black cap.
7. Stop solution,7 mL, yellow cap.
8. 20×concentrated washing buffer, 40 mL, white cap.
9. 2×concentrated redissolving solution 50 mL, transparent cap.
10. 2-Nitrobenzaldehyde, 10 mL, white cap.

Packaging:

96 Tests/Kit

Recovery Rate:

Tissue: 75±25%
Honey: 70±20%
Egg: 95±25%

Sample:

Tissue, Egg, Honey.

Sensitivity:

0.02 ppb

Storage Conditions:

Store at 2-8°C.

Tags:

Veterinary residue

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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