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Nitric Oxide Microplate Assay Kit

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Description:

This kit allows you to measure total nitric oxide (NO) produced for in vitro experimental systems following conversion of nitrate to nitrite by metallic cadmium. Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem involves the conversion of nitrate to nitrite catalyzed by cadmium. This is then followed by the quantitation of nitrite using the griess reaction.

Application:

Functional Assay

Shelf Life:

12 months

Cross Reactivity:

All

Intended Use:

This kit is suitable for the quantitative determination of total nitric oxide in samples that have high protein concentrations. The cadmium catalyst proves far more robust when in the presence of harsh deproteinating reagents than the alternative, nitrate reductase.

Packaging:

1 Kit

Storage Conditions:

Store at -20°C.

Tags:

Nitric Oxide Microplate Assay Kit

Target:

Nitric Oxide

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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