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Nitric Oxide Fluorometric Assay Kit

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Application:

Detection/Quantition

Shelf Life:

12 months

Advantages:

1.Simple procedure,takes only 2-3 hours.
2. Fast and convenient.
3. The fluorometric kit provides a simple two-step process for quantitative measurement of total nitrate/nitrite productions.
4.The assay can be easily done in a 96-well plate.

Cross Reactivity:

Mammal

Detection Method:

Fluorometric

Introduction:

Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. Since NO is rapidly converted to nitrite (NO²ˉ) and nitrate (NOᶟˉ), the total concentration of nitrite and nitrate is used as a quantitative measure of NO production. BioVision’s Nitric Oxide Fluorometric Assay Kit provides an accurate and convenient measurement of total nitrate/nitrite concentration in a simple two-step process. In the first step nitrate is converted to nitrite by nitrate reductase. In the second step, nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The fluorescent intensity is proportional to the total nitric oxide production. The kit has been tested with culture media, plasma, and tissue homogenates

Kit Contents:

1. Assay Buffer
2. Enzyme Cofactor
3. Enhancer
4. Nitrate Reductase
5. Nitrate Standard
6. Nitrite Standard
7. DAN Probe
8. Sodium Hydroxide
9. Microtiter plate
10. Plate Cover

Measurement:

Fluorometric (Ex/Em 380/461 nm)

Packaging:

200 assays

Sample:

Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Storage Conditions:

Store at -20°C.
avoid freeze/thaw cycles

Tags:

Nitric Oxide Fluorometric Assay Kit

Target:

Nitric Oxide

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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