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Nitric Oxide Colorimetric Assay Kit

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Price:

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Application:

Detection/Quantition

Shelf Life:

12 months

Advantages:

1. Simple two-step procedure: takes only ~1-1.5 hours.
2. Fast and convenient.
3. Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite.

Cross Reactivity:

Mammal

Detection Method:

Colorimetric

Intended Use:

Nitric Oxide Fluorometric Assay Kit

Introduction:

Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. NO is rapidly oxidized to nitrite and nitrate which are used to quantitate NO production. BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. The first step converts nitrate to nitrite utilizing nitrate reductase. The second step uses Griess Reagents to convert nitrite to a deep purple azo compound. The amount of the azochromophore accurately reflects nitric oxide amount in samples. The detection limit of the assay is approximately 0.1 nmole nitrite/well, or 1 µM

Kit Contents:

1. Assay Buffer
2. Enzyme cofactor
3. Enhancer
4. Nitrate Reductase
5. Nitrate Standard
6. Nitrite Standard
7. Griess Reagent R1
8. Griess Reagent R2
9. Microtiter Plate
10. Plate Cover

Measurement:

Colorimetric (540 nm Absorbance)

Packaging:

200 assays

Sample:

Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids

Storage Conditions:

Store at -20°C.

Tags:

Nitric Oxide Colorimetric Assay Kit

Target:

Nitric Oxide

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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