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MycoProbe Mycoplasma Detection Kit

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Description:

Mycoplasma contamination affects up to 80% of continuous cell cultures. If undetected, mycoplasma contamination can have significant effects on the quality and reliability of your cell culture preparations.

Mycoplasma contamination:

Induces deleterious effects on cell culture quality.
Alters the phenotypic characteristics of host cells.
Increases experimental variation.
Is common in eukaryotic cell cultures.
Is resistant to antibiotics such as penicillin and streptomycin.
Has multiple sources (i.e. personnel, reagents, other infected cells).

Shelf Life:

12 months

Advantages:

1. Reduces experimental variation.
2. Accurate and highly sensitive.
3. Simple multiwell-based colorimetric assay.
4. Only takes 4.5 hours.

Detection Principle:

Mycoplasma Detection in Cell Line Supernates and Cell Lysates. The presence of the eight mycoplasma species known to cause 95% of eukaryotic cell culture contamination was tested in supernates and cell lysates of the indicated cell lines using the MycoProbe Mycoplasma Detection Kit (Catalog # CUL001B). The average of the duplicate optical density (OD) readings for each control and sample was determined. The average negative control OD value was subtracted from all average sample OD values. A calculated positive control OD value of >0.10 indicated mycoplasma contamination (black line) in the CTLL-2, BaF3, A431, and K562 (cell lysate 1) samples. Abbreviations: CTLL-2 mouse cytotoxic T cell line, BaF3 mouse pro-B cell line, HepG2 human hepatocellular carcinoma cell line, A431 human epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line.

Intended Use:

A complete kit to detect common antibiotic-resistant cell culture contaminants

Kit Contents:

1. Cell Lysis Diluent Concentrate - 2 vials (1.7 mL/vial) of a 10-fold concentrated solution.
2. Hybridization Plate - One 96 well polystyrene microplate.
3. Streptavidin Plate - One 96 well polystyrene microplate (12 strips of 8 wells) coated with streptavidin.
4. Sample Diluent - 2 vials (21 mL/vial) of a buffered protein solution with preservatives.
5. Anti-digoxigenin Conjugate - 21 mL of a polyclonal antibody against digoxigenin conjugated to alkaline phosphatase with preservatives.
6. Capture Probes - 1.1 mL of a six-fold concentrated stock solution.
7. Detection Probes - 1.1 mL of a six-fold concentrated stock solution.
8. Positive Control - 1.1 mL of a solution containing a synthetic DNA oligonucleotide.
9. Wash Buffer Concentrate - 100 mL of a 10-fold concentrated solution with preservatives.
10. Substrate - 1 vial of lyophilized NADPH with stabilizers
11. Substrate Diluent - 1 vial (7 mL) of a buffered solution with stabilizers.
12. Amplifier - 1 vial of lyophilized amplifier enzymes with stabilizers.
13. Amplifier Diluent - 1 vial (7 mL) of a buffered solution containing INT-violet with stabilizers.
14. Stop Solution - 6 mL of 2 N sulfuric acid.
15. Float Collar - Microplate float collar for water bath.
16. Plate Sealers - 12 adhesive strips.

Packaging:

1 Kit

Storage Conditions:

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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