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Mycoplasma Test Kits

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Description:

PCR has been shown to be a highly sensitive, specific and rapid method for the detection of mycoplasmas contamination in cell cultures. Specific primers have been designed from DNA sequences that are coding for highly conserved ribosomal RNAs (16S-rRNA). The gene sequences for those conserved rRNAs are very similar in the various mycoplasma species and have not undergone significant mutations. Consequently, primers can be designed for these areas, which are specific to the contaminating mycoplasmas only and will not detect bacterial or animal DNA sequences.
PCR-based mycoplasma test kits greatly simplify testing and detection of mycoplasma contamination in cell cultures. With PCR testing, reliable results are obtained within a few hours, since the presence of contaminant mycoplasmas can be easily and sensitively detected by simply verifying the bands of amplified DNA fragments after gel electrophoresis.

Shelf Life:

12 months

Advantages:

1. Rapid – results within 5 hours.
2. Simple, one-step reaction – less than 10 minutes to prepare the sample. The mixture contains everything (including Taq Polymerase!)
3. Can be used routinely in every lab.
4. Minimal sample handling reducing the risk of contamination.
5. Highly sensitive (~10-1000 CFU/ml)*
6. M. hyorhinis can be detected

Intended Use:

PCR kits for sensitive and rapid detection of mycoplasmas in cell culture

Introduction:

The testing required by the regulatory authorities is seeding in culture (agar and liquid media). This test is complicated, time consuming (about 5 weeks), and some mycoplasma species are difficult to detect with this method. In recent years, the disadvantages of these methods have been acknowledged (such as sensitivity, specificity and long and complex procedures), and use of PCR for the detection of contaminations in cell cultures has become increasingly widespread.
(1) PCR Mycoplasma Test Kit I/C
(2) PCR Mycoplasma Test Kit I/RT
(3) PCR Mycoplasma Test Kit II

Storage Conditions:

Store at -20 °C
avoid freeze/thaw cycles

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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