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Mustard ELISA Kit

Product Name:

Mustard ELISA Kit

Price:

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Description:

Mustard is a condiment which can be prepared from mustard seeds and these can be classified into Brassicaceae (Cruciferae): Brassica juncea (brown), Brassica nigra (black), Sinapsis alba (yellow) varieties. It is often used in many different kinds of sauces and seasonings, this can result in masked allergen which can lead to allergic reactions. Also, due to high cross-reactivity this assay is suitable for analysing black and brown mustard. For a large number of mustard allergic people any hidden mustard allergens in food can cause them critical problems, there is evidence to indicate that even a very low amounts of mustard can lead to allergic reactions. This means that a mustard allergic individual must strictly avoid eating mustard or mustard containing food. Diagnostic procedures are often using prick-tests for mustard seeds and the detection of allergy antibodies is usually performed using blood samples.

Shelf Life:

12 months

Cross-reaction Rate:

Brown mustard (59%), Rapeseed (59%), Black mustard (50%), Charlock mustard (48%).

Detection Limit:

1.0 ppm

Detection Method:

ELISA

Incubation Time:

60 minutes

Intended Use:

Mustard ELISA kit is designed for measuring quantitative amounts of mustard residues present in food (such as cheese, sausage, mixed herbs, dressings and soups). This assay has a minimum analytical sensitivity limit of 1.0 ppm.

Kit Contents:

1. Microtiter Plate: Coated with anti mustard antibodies. 2. Mustard Standards 1-5: Concentration 0, 2, 6, 20, 60 ppm. 3. Conjugate (Anti Mustard Peroxidase). 4. Substrate Solution (TMB). 5. Stop Solution (0.5 M H₂SO₄). 6. Extraction and sample dilution buffer (Tris)(10x). 7. Washing Solution (10x Concentrate). 8. Instruction Manual.

Packaging:

96 well ELISA microplate

Precision:

0.08

Range of Quantitation:

2.0 ppm

Recovery Rate:

Sausage (98%), Canned soup (96%), Cheese (89%), Instant soup (80%), Mixed herbs (78%), Salad dressing (76%).

Sample:

Food (Mixed Herbs, Soups, Cheese, Dressings, Sausage)

Sample Size:

100 µL

Sensitivity:

The minimum detection sensitivity level of mustard residues using current mustard ELISA kit was 1.0 ppm. The standard range for this assay is 2.0-60.0 ppm.

Storage Conditions:

All reagents supplied need to be stored at 2-8°C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Substrate:

TMB 450 nm

Tags:

Food Analytic / Safety

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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