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Mollusc (Tropomyosin) ELISA Kit

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Description:

Molluscs represent a large group of seafood’s which can include mussels, squid, snails, abalone, oysters, octopus, scallops, clams and squid. It is also a food allergen since a large number of adverse reactions have been reported following the ingestion of molluscs, the allergic reactions found are similar to those found with other foods. Tropomyosin is a vital protein which is common to all mollusc species, it represents a high amount of the total protein present for other cooked or uncooked mollusc extracts.
A very small quantity of allergen is enough cause allergic reactions and in severe cases can result in anaphylactic shock. There is also the possibility of cross-reactions between molluscs in particular within the same class (for example cephalopods, bivalves and gastropods). Allergies to molluscs tend to be diagnosed in a similar fashion to other food allergies.

Shelf Life:

12 months

Detection Limit:

1.7 ppb

Detection Method:

ELISA

Intended Use:

Mollusc (tropomyosin) ELISA kit can be used for detecting quantitative levels of tropomyosin and mollusc in food (such as fish, meat, bakery products, soups and dressings). This assay has a minimum analytical sensitivity limit of 1.7 ppb.

Kit Contents:

1. Microtiter Plate: Coated with anti tropomyosin antibodies.
2. Tropomyosin Standards 1-5: Concentration 0, 10, 40, 100, 400 ppb.
3. Conjugate (Anti Tropomyosin Peroxidase).
4. Substrate Solution (TMB).
5. Stop Solution (0.5 M H₂SO₄).
6. Extraction and sample dilution buffer (Tris)(10x).
7. Washing Solution (PBS + Tween 20)(10x Concentrate).
8. Instruction Manual.

Packaging:

96 well ELISA microplate

Precision:

5-6%

Range of Quantitation:

10.0 ppb

Sample:

Food (Soups, Fish, Bakery, Dressings, Meat)

Sensitivity:

The minimum detection sensitivity level of mollusc and tropomyosin using current mollusc/tropomyosin ELISA kit was 1.7 ppb. The standard range for this assay is 10.0-400.0 ppb.

Storage Conditions:

All reagents supplied need to be stored at 2-8°C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Tags:

Food Analytic / Safety

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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