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Lysozyme ELISA Kit

Product Name:
Lysozyme ELISA Kit
Price:
Description:
Lysozyme (1,4-β-N-acetylmuramidase) is an antimicrobial enzyme that is vital in the prevention of bacterial infections and it is produced by animals as part of the innate immune system. Reduced levels of lysozyme have also been linked to bronchopulmonary dysplasia in newborns. It is abundant in secretions such as saliva, mucus, tears and human milk. In humans, hen’s egg (also called Gallus gallus) provides a rich food source, where the proteins of the egg yolk display minor allergenicity and the egg white proteins are known to be allergenic.
For an individual that is allergic, even small amounts of consumption of lysozyme can cause an allergic reactions and in some case could lead to severe anaphylactic shock. In addition to ovotransferrin, livetin, ovalbumin and ovomucoid, lysozyme represents a very important allergen.
Shelf Life:
12 months
Cross-reaction Rate:
Egg white protein, total (2.2%), Ovalbumin, Conalbumin, Ovomucoid (< 0.0001%), Chicken (< 0.000006%), Cashew (< 0.000005%).
Detection Limit:
2.0 ppb
Detection Method:
ELISA
Intended Use:
Lysozyme ELISA kit is intended for detecting quantitative concentrations of lysozyme residues in food (such as wine or cheese products). This assay has a minimum analytical sensitivity limit of 2.0 ppb.
Kit Contents:
1. Microtiter Plate: Coated with anti-lysozyme antibodies.
2. Lysozyme standards 1-5: Concentration 0, 25, 50, 100, 250 ppb.
3. Conjugate (Anti-Lysozyme-Peroxidase).
4. Substrate Solution (TMB).
5. Stop Solution (0.5 M H2SO4).
6. Extraction And Sample Dilution Buffer (Tris)(10x).
7. Washing Solution (PBS + Tween 20)(10x Concentrate).
8. Instruction Manual.
Packaging:
96 well ELISA microplate
Range of Quantitation:
25.0 ppb
Recovery Rate:
Wine (99%), Cheese (85%).
Sample:
Food (Wine, Cheese)
Sensitivity:
The minimum detection sensitivity level of lysozyme residues using current lysozyme ELISA kit was 2.0 ppb. The standard range for this assay is 25.0 – 250.0 ppb.
Storage Conditions:
All reagents supplied need to be stored at 2-8°C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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