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Lupine ELISA Kit

Product Name:

Lupine ELISA Kit

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Description:

Lupine (also referred to as lupin or lupinus) belongs to the genus of legume family, which includes over 200 different species. The lupin seed contains a high fraction of proteins (approx. 35-45%) and these proteins (mainly α-, β- and γ-conglutin fractions) usually lead to allergic reactions because of their cross reactivity to allergens of peanut and soya. Lupine is frequently in dietary products, in particular for individuals that are allergic to milk, patients with celiac disease and vegetarians.
Due to high cross-reactivity, the following assay is suitable for measuring all commonly used lupine species. During many food production processes there are three sweet lupine species which are often used: Lupinus angustifolius, Lupinus luteus and Lupinus albus. The presence of lupine allergins in foods can lead to critical problems to people that are allergic to lupin. Even very low levels can result in an allergic reaction and in severe cases can lead to anaphylactic shock.

Shelf Life:

12 months

Cross-reaction Rate:

Soy flour (unroasted)(0.07%), Soy lecithin (0.002%), Soy flour (roasted) (0.0009%), Lentil (0.0004%), Chickpea (0.0003%), Hazelnut (0.0002%).

Detection Limit:

0.02 ppm

Detection Method:

ELISA

Intended Use:

Lupine ELISA kit is designed for analysing quantitative amounts of lupine (lupin and lupinus) in food (such as sausage, ketchup, potato products, juices and bakery products). This assay has a minimum analytical sensitivity limit of 0.2 ppm.

Kit Contents:

1. Microtiter Plate: Coated with anti lupin antibodies.
2. Lupin Standards 1-5: Concentration 0, 2, 5, 15, 30 ppm.
3. Conjugate (Anti Lupin Peroxidase).
4. Substrate Solution (TMB).
5. Stop Solution (0.5 M H₂SO₄).
6. Extraction and sample dilution buffer (Tris)(10x).
7. Washing Solution (PBS + Tween 20)(10x Concentrate).
8. Instruction Manual.

Packaging:

96 well ELISA microplate

Precision:

6-9%

Range of Quantitation:

2.0 ppm

Recovery Rate:

Bread (113%), Croquette (111%), Orange juice (104%), Sausage (99%), Ketchup (98%).

Sample:

Food (Bakery, Sausage, Potato, Ketchup, Juices)

Sensitivity:

The minimum detection sensitivity level of lupin and lupinus derivatives current lupine ELISA kit was 0.2 ppm. The standard range for this assay is 2.0-30.0 ppm.

Storage Conditions:

All reagents supplied need to be stored at 2-8°C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Tags:

Food Analytic / Safety

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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