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Global RNA Synthesis Assay Kit II

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Description:

Global RNA Synthesis Assay Kit II provides a simple and robust tool for detection of global RNA transcription temporally and spatially or changes in RNA levels directly in living cells. De novo synthesized RNA can be detected with a simple procedure without the use of radiolabeling or antibodies. Our approach relies on the incorporation of cell permeable 5-EU (Ethynyl uridine) into nascent RNA, but not into DNA, instead of its natural uridine analog. 5-EU can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo synthesized RNA in proliferating cells. Modified RNA is detected by click chemistry with azide-containing dye that enables for multiplex analyses with other probes, or detection of RNA-interactive proteins for deeper biological insights. Our kit provides sufficient materials for 100 assays for analysis by FACS or detection by fluorescence microscope. We include Actinomycin D, an inhibitor RNA synthesis that serves as an experimental control.

Application:

Fast and simple cell-based assay to detect RNA transcription in biological samples using fluorescence microscopy or flow cytometry (red fluorescence).

Shelf Life:

12 months

Packaging:

100 assays

Storage Conditions:

Store at -20°C.

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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