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Global Protein Synthesis Assay Kit II

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Description:

Global Protein Synthesis Assay Kit II utilizes a novel and robust chemical method based on an alkyne containing and cell-permeable analog of puromycin, O-Propargyl-puromycin (OP-puro). Once inside the cell, OP-puro stops translation by forming covalent conjugates with nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide. Unlike methionine analogs, OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture. Our kit provides sufficient materials for 100 assays to detect nascent proteins synthesized under various physiological conditions, and Cycloheximide, an inhibitor of protein synthesis that serves as an experimental control.

Application:

Fast and simple cell-based assay to accurately measure de novo protein synthesized in biological samples using fluorescence microscopy or flow cytometry (green fluorescence)

Shelf Life:

12 months

Packaging:

100 assays

Storage Conditions:

Store at -20°C.

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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