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Global Protein Synthesis Assay Kit I

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Description:

Global Protein Synthesis Assay Kit I utilizes a novel chemical method based on alkyne analog of puromycin, O-Propargyl-puromycin (OP-puro). OP-puro stops translation by forming covalent conjugates with the nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide (Ex/Em = 494/521 nm). OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture medium. We provide sufficient materials for 100 simple and specific assays to detect nascent proteins synthesized under various physiological conditions in real-time, and in the presence of Cycloheximide, an inhibitor of protein synthesis that serves as a control.

Application:

Simple, rapid, fluorometric plate-based assay for accurately measuring de novo protein synthesized in biological samples.

Shelf Life:

12 months

Packaging:

100 assays

Storage Conditions:

Store at -20°C.

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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