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Flow Cytometric Apoptosis Detection Kit (Fluorometric)

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Flow Cytometric Apoptosis Detection Kit (Fluorometric)

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Description:

Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D2R), a reported substrate for members of caspase family proteases. The caspase substrate D2R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.

Application:

Flow Cytometry

Shelf Life:

6-12 months

Advantages:

1. D2R is more cell-permeable than other fluorometric Caspase substrates. 2. Fast and convenient. 3. Simple one-step procedure. 4. Takes only 30 minutes.

Assay Type:

Apoptosis Detection

Kit Contents:

1. D2R Reagent. 2. DTT (1 M). 3. D2R Incubation Buffer.

Other Instruments Required:

Flow cytometer

Storage Conditions:

Shipped -20°C Dry Ice, Store at -20°C. Avoid freeze-thaw cycles.

Tags:

Apoptosis

Target:

Apoptosis

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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