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Doxycycline ELISA Kit

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Description:

The Doxycycline ELISA kit is an indirect competitive assay to quantitatively detect the presence of Doxycycline in seafood samples.

Shelf Life:

12 months

Detection Principle:

This test kit is based on the solid phase enzyme-linked immunosorbent assay (ELISA) for the detection of Doxycycline residue. An unknown amount of Doxycycline present in the sample and the fixed amount of Doxycycline antigens pre-coated on the wells of microtiter plate/strips compete for the anti-Doxycycline antibodies, which in turn is detected with enzyme conjugate. After incubation the wells are washed and the bound enzyme is visualised by adding TMB solution. Any coloured product is measured at 450 nm after adding stop solution. The absorbance value of the developed colour is inversely proportional to the amount of the Doxycycline in the sample. The quantity of Doxycycline in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Introduction:

Doxycycline (DOX) is a semi-synthetic broad-spectrum antibiotic, active against a wide variety of bacteri

Kit Contents:

1. 1 x Pre-coated Microtiter plate (12 x 8 microwells).
2. 1 x Doxycycline Enzyme conjugate (7 ml).
3. 1 x Doxycycline Antibody solution (7 ml).
4. 1 x Wash buffer concentrate (20×, 30 ml).
5. 5 x Doxycycline standards (25×, 0.3 ml/each) (0 ppb, 10 ppb, 30 ppb, 90 ppb, 270 ppb).
6. 1 x Doxycycline high concentrate standard (0.3 ml).
7. 1 x TMB solution (15 ml).
8. 1 x Assay buffer concentrate (20×, 10 ml).
9. 1 x Stop solution (15 ml).
10. 1 x Package insert.
11. 1 x Microplate sealer.

Packaging:

1 x 96 well Plate

Storage Conditions:

Store at 2-8°C

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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