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Domoic Acid (ASP) ELISA Kit

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Description:

Immunoassay for the quantitative and sensitive detection of Domoic Acid (ASP); AOAC Method 2006.02. Domoic Acid is one of the toxins associated with amnesiac shellfish poisoning (ASP). This test is suitable for the quantitative and/or qualitative detection of Domoic Acid in water samples as well as shellfish samples. for shellfish samples a sample preparation is required. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.

Detection Method:

The assay is in a direct competition format, where free Domoic Acid (DA) in the sample competes with DA-conjugated protein coated on plastic wells for binding to anti-DA antibodies free in the solution. The polyclonal ovine anti-DA antibodies are conjugated to horseradish peroxidase (HRP). Sample diluted in buffer is incubated in the wells with the anti-DA-antibody-HRP conjugate. After washing, the amount of conjugate remaining bound to the well is measured by incubation with a substrate that gives a blue product upon reaction with the HRP enzyme. Addition of acid stops the reaction and changes the product colour from blue to yellow. The color intensity is measured spectrophotometrically on a plate-reader at 450 nm, and is inversely proportional to the concentration of DA in the sample solution. The assay is calibrated using dilutions of a DA calibration solution supplied with the kit. The calibrated range of the assay (I20 - I80) is approximately 10 to 300 pg/mL of DA. The working range for ASP toxins in shellfish is 0.01mg/kg up to at least 250 mg/kg.

Packaging:

96-Well Plate ELISA

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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