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DNA Fragmentation Detection Kit

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Description:

Cell death occurs by two major mechanisms, necrosis and apoptosis. Apoptosis is also known as programmed cell death or anoikis (a form of apoptosis which is induced by anchorage-dependent cells detaching from the surrounding extracellular matrix). Apoptosis leads to the elimination of cells without releasing harmful substances into the surrounding area. Too little or too much apoptosis plays a role in a great many diseases. When apoptosis functions inappropriately, cells that should be eliminated survive and potentially become immortal, as in cancer or leukemia. When apoptosis works overly well, too many cells may ‘die’ and the result may be grave tissue damage. This is the case in stroke and neurodegenerative disorders such as Alzheimer, Huntington and Parkinson diseases. The term ‘apoptosis’ refers only to the structural changes a cell goes through during the process of programmed cell death and not to the process itself. Classical necrotic cell death occurs due to noxious injury or trauma to the cell while apoptosis is an energy dependent mechanism that takes place during normal cell development. While necrotic cell death results in cell lysis, cellular apoptosis is characterized morphologically by cell shrinkage, nuclear pyknosis, chromatin condensation, and blebbing of the plasma membrane. Apoptosis is the result of a cascade of molecular and biochemical events involving endogenous endonucleases that cleave DNA into the prototypical ‘ladder of DNA fragments’ that may be visualized in agarose gels. Observation of oligonucleosomal DNA fragments by DNA laddering has long been the most acceptable and only available assay for the detection of apoptosis. DNA Fragmentation Detection Kit exploits the fact that apoptotic endonucleases not only affect cellular DNA by producing the classical DNA ladder but also generate free 3’-OH groups at the ends of these DNA fragments. These free 3’-OH groups are end-labeled by the DNA Fragmentation Detection Kit allowing for the detection of apoptotic cells using a molecular biology-based, end-labeling technique.

Application:

Microscopy (visible)

Shelf Life:

12 months

Assay Type:

Detection/Quantition

Kit Contents:

1. Proteinase K. 2. TdT Equilibration Buffer. 3. TdT Labeling Reaction Mix. 4. TdT Enzyme. 5. Stop Buffer. 6. Block Buffer. 7. Streptavidin-HRP Conjugate (25X). 8. DAB Concentrate. 9. DAB Reaction Buffer. 10. Methyl Green Counterstain.

Other Instruments Required:

Microscope (visible)

Sample:

Cell Cultures, Tissues

Storage Conditions:

Shipped +4°C Ice Packs, Store at -20°C.

Tags:

Apoptosis

Target:

Apoptosis

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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