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Diazepam ELISA Test Kit

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Description:

Diazepam is an anxiolytic-sedative drug useful in the symptomatic relief of anxiety and tension states.

Shelf Life:

18 months

Cross-reaction Rate:

Urine：80 ±10%. Feed: 75±10%. Tissue (meat, liver): 85±10%.

Detection Limit:

Tissue: 1 ppb. Urine：1 ppb. Feed：10 ppb.

Detection Principle:

The Diazepam ELISA Test Kit is based on the competitive enzyme immunoassay for the detection of Diazepam in the feed, urine, liver, meat. The coupling antigen is pre-coated on the micro-well stripes. The Diazepam in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Diazepam antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Diazepam in it. This value is compared to the standard curve and the Diazepam concentration is subsequently obtained.

Introduction:

Diazepam was invented by Stembach of Hoffmann-La roche. Diazepam is commonly prescribed as a medication to relief anxiety for a
short period. It is also prescribed to treat neurology conditions such as some types of epilepsy, some forms of paresis, and a rare disorder called stiff-person syndrome.

Kit Contents:

1. Micro-well coated strip plate (12 strips with 8 removable wells each).
2. 6x standard solution (1 mL each): 0.0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb.
3. Enzyme conjugate (7 mL) DE-100183.
4. Antibody working solution (10 mL) DE-100184.
5. Substrate A solution (7 mL) DE-SSA.
6. Substrate B solution (7 mL) DE-SSB.
7. Stop solution (7 ml) DE-ST.
8. 20x concentrated washing buffer (40 mL) DE-WB.
9. 2x concentrated redissolving solution (50 mL) DE-SS2.
10. Instruction Manual.

Packaging:

96 Test/Kits

Recovery Rate:

Diazepam: 100%. Nitrazepam: 7.6%. Oxazepam: 8.8%.

Sample:

Animal tissues(meat, liver), Urine, Feed.

Sensitivity:

0.1 ppb

Storage Conditions:

Store at 4-30°C.

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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