Home
Products
Drug Discovery Test Kits
Cell Apoptosis Test Kits
Cytochrome c Releasing Apoptosis Assay Kit (Western Blot)

Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form at the bottom. We will get back to you as soon as possible.

Cytochrome c Releasing Apoptosis Assay Kit (Western Blot)

Product Name:

Price:

Inquiry

Description:

Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. The Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit.

Application:

Western blot

Shelf Life:

12 months

Advantages:

1. Cytochrome C releasing from mitochondria into cytosol is determined by Western blotting using the Cytochrome C antibody provided in the kit.
2. Fast and convenient.
3. No ultracentrifugation required.
4. Simple and straightforward procedure.
5. Takes-3 hours.

Assay Type:

Detection/Quantition

Detection Method:

Western blot

Intended Use:

Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol.

Kit Contents:

1. Mitochondria Extraction Buffer.
2. 5X Cytosol Extraction Buffer.
3. DTT (1 M).
4. 500X Protease Inhibitor Cocktail.
5. Anti-Cytochrome c mouse mAb (0.2 mg/mL).

Other Instruments Required:

Western blotting apparatus

Species Reactivity:

Mammal

Storage Conditions:

Shipped -20°C Dry Ice, Store at -20°C. Avoid freeze-thaw cycles.

Target:

Apoptosis

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

Online Inquiry