Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form at the bottom. We will get back to you as soon as possible.

Cytochrome c Releasing Apoptosis Assay Kit

Product Name:

Price:

Inquiry

Description:

Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. The Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit.

Application:

Western blot

Shelf Life:

Avoid freeze-thaw cycles, 12 months shelf life.

Advantages:

1. Cytochrome C releasing from mitochondria into cytosol is determined by Western blotting using the Cytochrome C antibody provided in the kit.
2. Fast and convenient
3. No ultracentrifugation required
4. Simple and straightforward procedure
5. Takes ~3 hours

Assay Type:

Detection/Quantition

Detection Method:

Western blot

Intended Use:

Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol.

Kit Contents:

1. Mitochondria Extraction Buffer
2. 5X Cytosol Extraction Buffer
3. DTT (1 M)
4. 500X Protease Inhibitor Cocktail
5. Anti-Cytochrome c mouse mAb (0.2 mg/mL)
(See Datasheet for specific volumes supplied)

Species Reactivity:

Mammal

Storage Conditions:

Shipped -20°C Dry Ice, Store at -20°C.

Tags:

Apoptosis

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

Online Inquiry