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Crustacean (Tropomyosin) ELISA Kit

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Description:

Crustacean form part of eight other food groups which can trigger allergies by the presence of immunoglobulin E antibodies. This usually leads to hypersensitivity reactions due to ingesting these crustaceans (examples include lobsters, shrimps and crabs), these are the most frequent causes of many food allergic reactions. Also, crustaceans are regarding as the third important foodstuff which can induce food related anaphylaxis. One of the major allergen found in shrimps and many other crustaceans is the ubiquitous muscle protein tropomyosin, this is also responsible for a large number of the ingestion related allergic reactions. It is a highly conserved protein and is known to be homologous in a large number of different species (displaying considerable rate of identicalness). Tropomyosin is relatively resistant to heat and acidic digestion. The heat stability property allows this protein to be suitable for analysing in processed food samples.
Some of the detection methods for food allergens (especially crustacean protein) need to be highly specific and be able to detect minute quantities of the allergen which could be present in complex food matrices. Currently, there are a few procedure available (PCR, lateral flow assays, ELISA) but not many are able to detect tropomyosin protein.

Shelf Life:

12 months

Cross-reaction Rate:

Cockroach (protein) (0.01%)

Detection Limit:

0.9 ppm

Detection Method:

ELISA

Intended Use:

Crustacean (tropomyosin) ELISA kit is a procedure designed for detecting quantitative amounts of crustacean or tropomyosin in food products (such as fish, meat, bakery and soups). This assay has a minimum analytical sensitivity limit of 0.09 ppm.

Kit Contents:

1. Microtiter Plate: Coated with anti-tropomyosin antibodies.
2. Tropomyosin Standards 1-5: Concentration 0, 20, 60, 200, 400 ppb.
3. Conjugate (Anti-Tropomyosin-Peroxidase).
4. Substrate Solution (TMB).
5. Stop Solution.
6. Extraction and sample dilution buffer (Tris)(10x Concentrate).
7. Washing Solution (PBS + Tween 20)(10x).
8. Instruction Manual.

Packaging:

96 well ELISA microplate

Precision:

6-8%

Range of Quantitation:

20.0 ppm

Recovery Rate:

Meat (97%), Fish (93%), Vegetable soup (93%), Bakery products (90%), Soy sauce (84%).

Sample:

Food (Bakery, Fish, Meat, Soups)

Sensitivity:

The minimum detection sensitivity level of crustacean (tropomyosin) using current crustacean/tropomyosin ELISA kit was 0.09 ppm. The standard range for this assay is 20.0-400.0 ppm.

Storage Conditions:

All reagents supplied need to be stored at 2-8°C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Tags:

Food Analytic / Safety

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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