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Chlortetracyline ELISA Test Kit

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Shelf Life:

12 months

Cross-reaction Rate:

Chlortetracyline：100%
Tetracyclines： 80%
Oxytetracyline： 40%
Doxycycline： 10%

Detection Limit:

Tissue: 3 ppb
Honey: 5 ppb

Detection Principle:

This test kit is based on the competitive enzyme immunoassay for the detection of Chlortetracyline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Chlortetracyline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Chlortetracyline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chlortetracyline in it. This value is compared to the standard curve and the Chlortetracyline concentration is subsequently obtained.

Kit Contents:

1. Micro-well strips: 12 strips with 8 removable wells each.
2. 10×concentrated standard solution (0.5ml/bottle): 0 ppb, 2 ppb, 6 ppb, 18 ppb, 54 ppb.
3. Enzyme conjugate (7 mL).
4. Antibody working solution (7 mL).
5. Substrate A (7 mL).
6. Substrate B (7 mL).
7. Stop solution (7 mL).
8. 20×concentrated washing buffer (30 mL).
9. 20×concentrated redissolving solution (10 mL).

Packaging:

96 Tests/Kit

Recovery Rate:

Tissue: 90±15%

Sensitivity:

0.2 ppb

Storage Conditions:

Store at 2-8°C.

Tags:

Veterinary residue

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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