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CellGreen Cell Proliferation Kit

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Description:

The CellBlue and CellGreen Proliferation Dyes diffuse passively into live cells and are used for long-term cell labeling. They are initially non-fluorescent esters but are converted to fluorescent dyes by intracellular esterases. The dyes then covalently react with amine groups on proteins, forming fluorescent conjugates that are retained in the cell. Immediately after staining a single, bright fluorescent population will be detected by flow cytometry. Each cell division that occurs after labeling results in the appearance of a dimmer fluorescent peak on a flow cytometry histogram (Fig. 1). Cell proliferation dyes can be used to track cell divisions in vivo or in vitro. Staining can withstand fixation and permeabilization for subsequent immunostaining. In addition, the CellGreen Cell Proliferation Dye is more fixable than CFSE.
The CellBlue and CellGreen dyes were developed to provide superior cell staining, fixability, and low toxicity. The CellBlue dye 405 is detected in the Pacific Blue® channel and gives sharp peaks with no toxicity (Fig. 1). The CellGreen dye is detected in the FITC channel, but is less toxic, less leaky and more fixable than the classic dye CFSE. It also has less bleed-through into other channels such as PE.
Alternative applications of cell proliferation dyes include uniform labeling of cell cytoplasm for microscopy, or labeling cells for quantitation of cell number by microplate reader (note: detection by microplate reader can only be used to quantitate total cell number immediately after staining with cell proliferation dyes, not to track cell divisions).

Application:

For long-term cell imaging by fluorescence microscopy and tracking cell proliferation in vivo or in vitro by flow cytometry

Shelf Life:

12 months

Packaging:

100 assays/1000 assays

Storage Conditions:

Store at -20°C.

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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