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Cashew ELISA Kit

Product Name:

Cashew ELISA Kit

Price:

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Description:

Cashew tree (Anacardium occidentale) is a tropical evergreen tree that belongs to the Anacardiaceae family and it produces cashew seed and cashew apple. These seed shells have been used to yields derivatives which are useful in many applications such as paints, waterproofing and lubricants. Cashews have a lower fat content when compared to most other nuts, approx. 82% is unsaturated fatty acids and around 66% of this is heart-healthy monounsaturated fats. Studies have indicated that people allergic to cashew are also allergic to pistachio (process called cross-reactivity), both of these nuts are botanically related. Cashew food allergies are usually linked to the presence of IgE antibodies which are directed against the major seed storage proteins in Cashew (namely 13S globulin proteins and 2S albumins). It has been found to frequently induce symptoms of food allergy, hypersensitivity reactions and atopic dermatitis in individuals that are sensitised.

Shelf Life:

12 months

Cross-reaction Rate:

Pistachio (4%)

Detection Limit:

0.2 ppm

Detection Method:

ELISA

Incubation Time:

60 minutes

Intended Use:

Cashew ELISA kit is designed for determining quantitative levels of cashew in food (such as chocolate, ice-cream, cereals and cookies). This assay has a minimum analytical sensitivity limit of 0.2 ppm.

Kit Contents:

1. Microtiter Plate: Coated with anti-cashew antibodies. 2. Cashew Standards 1-5: Concentration 0, 2, 6, 20, 60 ppm. 3. Conjugate (Anti-Cashew-Peroxidase). 4. Substrate Solution (TMB). 5. Stop Solution. 6. Extraction and sample dilution buffer (Tris)(10x Concentrate). 7. Washing Solution (10x). 8. Instruction Manual.

Packaging:

96 well ELISA microplate

Precision:

4-5%

Range of Quantitation:

2.0 ppm

Recovery Rate:

Cookies (109%), Dark Chocolate (102%), Cereals (98%), Ice-cream (93%).

Sample:

Food (Chocolate, Cookies, Ice Cream, Cereals)

Sample Size:

100 µL

Sensitivity:

The minimum detection sensitivity level of cashew using current cashew ELISA kit was 0.2 ppm. The standard range for this assay is 2.0-60.0 ppm.

Storage Conditions:

All reagents supplied need to be stored at 2-8°C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Substrate:

TMB 450 nm

Tags:

Food Analytic / Safety

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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