Product Name:
Brazil nut ELISA Kit
Description:
Brazil nut (Bertholletia excelsa) belongs to the Lecythidaceae family and is the only species in the montypic genus Bertholletia. It is often found in South America and is native to countries such as Brazil, Colombia, Bolivia, Venezuela, Peru and Guianas. This family is in the order Ericales, which includes some other more well-known plants for example cranberries, blueberries, tea, sapote, phlox, persimmons and gutta-percha.
Brazil nut allergies are usually more commonly found in adults rather than children, allergic reactions are frequently severe and also have the potential to cause anaphylaxis (such as breathing difficulty). Brazil nuts can be eaten raw and are often used in cakes, biscuits, bread, muesli, ice cream and confectionery. Also, people who are allergic to Brazil nuts have a unique problem becuase it is the only allergic reaction which can be sexually transmissible (i.e. the semen of a man who has eaten brazil nuts is able to trigger an allergic response if his sexual partner has an allergy).
Cross-reaction Rate:
Hazelnut (0.0007%), Pecan nut (0.0002%)
Incubation Time:
60 minutes
Intended Use:
Brazil nut ELISA kit is a method for detecting quantitative amounts of Brazil nut in food (such as ice-cream, cookies, chocolate, cereals and sausage). This assay has a minimum analytical sensitivity limit of 0.02 ppm.
Kit Contents:
1. Microtiter Plate: Coated with anti brazil nut antibodies.
2. Brazil nut Standards 1-5: Concentration 0, 1, 4, 10, 40 ppm.
3. Conjugate (Anti-Brazil Nut-Peroxidase).
4. Substrate Solution (TMB).
5. Stop Solution (0.5 M H2SO4).
6. Extraction and sample dilution buffer (TRIS)(10x Conc.).
7. Washing Solution (10x concentrate).
8. Instruction Manual.
Packaging:
96 well ELISA microplate
Range of Quantitation:
1.0 ppm
Recovery Rate:
Cornflakes (112%), Ice-cream (107%), Cookies (93%), Sausage (90%), Chocolate (76%).
Sample:
Food (Sausage, Chocolate, Ice Cream, Cereals, Cookies)
Sensitivity:
The minimum detection sensitivity level of Brazil nut using current Brazil nut ELISA kit was 0.02 ppm. The standard range for this assay is 1.0-40.0 ppm.
Storage Conditions:
All reagents supplied need to be stored at 2-8°C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
Tags:
Food Analytic / Safety
