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Beta-lactoglobulin ELISA Kit

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Description:

Beta-Lactoglobulin (beta-LG) is a protein which is present in majority of ruminant species and is found in the milk of many other species. It is main whey protein found in cow and sheep’s milk (approx. 3g/l), belonging to the lipocalins family that has the ability to bind a large number of hydrophobic molecules (indicating a potential transport function). Lipocalins proteins also contain molecular pockets that allow them to accommodate iron complexes (siderohores). Beta-lactoglobulin accounts for a significant proportion of cow’s milk allergy, especially those found in children. Very low amounts of bovine milk is found to cause an allergic reaction, in severe cases this can lead to anaphylactic shock. Also, the presence of hidden milk proteins for example in cookies, convenience food, sausage and beverages may cause critical problems to individuals that are allergic to milk.

Shelf Life:

12 months

Cross-reaction Rate:

Ewe’s milk (< 0.2%), Casein (< 0.02%), Goat’s milk (< 0.002%).

Detection Limit:

1.5 ppm

Detection Method:

ELISA

Incubation Time:

60 minutes

Intended Use:

Beta-lactoglobulin ELISA kit can be used to measuring quantitative amounts of ß-lactoglobulin (beta lactoglobulin, beta-LG, beta-lactoglobulin) in food samples (for example chocolate, cookies, cereals, soy products, sausage, wine and orange juice). This assay has a minimum analytical sensitivity limit of 1.5 ppm.

Packaging:

96 well ELISA microplate

Precision:

0.07

Range of Quantitation:

10 ppm

Recovery Rate:

Sausage (107%), Orange juice (98%), Cereals (94%), Cookies (88%), Chocolate (86%), White wine (82%), Soy milk (70%).

Sample:

Food (Soy Products, Chocolate, Cookies, Sausage, Cereals, Orange Juice, Wine)

Sample Size:

100 µL

Sensitivity:

The minimum detection sensitivity level of ß-lactoglobulin (beta-LG, beta lactoglobulin) using current beta-lactoglobulin ELISA kit was 1.5 ppm. The standard range for this assay is 10.0 – 400.0 ppm.

Storage Conditions:

All reagents supplied need to be stored at 2-8°C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Substrate:

TMB 450 nm

Tags:

Food Analytic / Safety

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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