Product Name:
Benzyl penicillin ELISA Kit
Description:
for Qualitative and Quantitative Determination of Benzylpenicillin in chicken, duck, pork, liver, fish, shrimp, honey, and milk.
Shelf Life:
12 months, please check date of production before use.
Cross-reaction Rate:
Benzyl penicillin: 100%
Ampicillin: 0.8%
Cloxacillin: 0.2%
Dicloxacillin: 0.1%
Amoxicillin: 0.1%
Ceftiofur: 0.1%
Detection Limit:
Chicken, duck, pork, liver, fish, shrimp: 2 ppb
Honey, milk: 2 ppb
Detection Principle:
This test kit is based on the competitive enzyme immunoassay for the detection of Benzylpenicillin in the chicken, pork, duck, honey, milk, fish, shrimp and egg, etc.
Introduction:
All penicillins are Beta-lactam antibiotics which in clinical use are the main active antibiotics against Gram-positive bacteria. Beta-lactam antibiotics are bactericidal, which act by inhibiting the synthesis of the peptidoglycan layer of the wall on the bacteria. Peptidoglycan is formed of sugars and amino acids that form the layer outside the plasma membrane of the bacteria cell wall. Penicillin interferes with the production of peptidoglycan by binding to bacterial enzymes known as transpeptidases.
Benzylpenicillin is also used in the treatment of animal disease to promote a healthy environment for the farming industry. However if the animals are treated with high doses of benzylpenicillin, its residues can cause allergic reaction and tolerance to the drug. Benzylpenicillin was banned in the EU and America.
Kit Contents:
1. Micro-well coated strip plate(12 strips with 8 removable wells each).
2. 6x standard solution (1 mL each):0.0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb.
3. Enzyme conjugate (12 mL).
4. Antibody working solution (7mL).
5. Substrate A solution (7 mL).
6. Substrate B solution (7 mL).
7. Stop solution (7 mL).
8. 20X Concentrated Washing buffer (40 mL).
9. 10X Concentrated redissolving solution (50 mL) DE-SS2.
10. Instruction Manual.
Recovery Rate:
Chicken, duck, pork, liver, fish, shrimp: 85±10%
Honey, milk: 70±10%
Sample:
Tissue (chicken, duck, fish, shrimp, pork or pork liver), Honey, Milk.
Storage Conditions:
Store at 2-8°C, not frozen.
