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Benzo(a)Pyrene ELISA Kit

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Description:

The Abraxis Benzo(a)Pyrene ELISA is an immunoassay for the quantitative and sensitive detection of Benzo(a)Pyrene in water samples. Sample concentration is not required prior to analysis for LOD of 0.3 ppb, if lower LOD are required a simple concentration step is necessary. If necessary, positive samples can be confirmed by HPLC or other conventional methods.

Cross-reaction Rate:

Benzo(a)Pyrene： 100% (per definition)
Chrysene： 77.6%
indeno{1,2,3-cd} Pyrene: 49.7%
Benzo(b) Fluoranthene: 20.2%
Benzo(a)Anthracene: 11.7%
Pyrene: 10.9%
Fluoranthene: 8.6%
Benzo(k) fluoranthene: 5.0%

Detection Principle:

The test is a direct competitive ELISA that allows the detection of Benzo(a)Pyrene.

Introduction:

B(a)P is a carcinogen known to induce tumors in a number of organs in animal models and humans. One of its metabolites is known to form DNA adducts which are responsible for mutagenesis and tumorigenesis.

Kit Contents:

1. Micro-well strips: 12 strips with 8 removable wells each.
2. 6×standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb.
3. Enzyme conjugate (12 mL) red cap.
4. Antibody working solution (7 mL) blue cap.
5. Substrate A solution (7 mL) white cap.
6. Substrate B solution (7 mL) black cap.
7. Stop solution (7 mL) yellow cap.
8. 20×concentrated washing buffer (40 mL) white cap.
9. 10×concentrated redissolving solution (50 mL) transparent cap.

Packaging:

96 Tests/Kit

Recovery Rate:

Coefficients of variation (CVs) for standards: <10%, for samples: < 20%

Sensitivity:

0.30 ppb (ug/L

Stability:

Solutions should be allowed to reach room temperature (20-25°C) before use. Reagents may be used until the expiration date on the
box. Consult state, local and federal regulations for proper disposal of all reagents.

Storage Conditions:

The Benzo(a)Pyrene ELISA should be stored in the refrigerator (4-8°C).

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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