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ApoDIRECT DNA Fragmentation Assay Kit

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Description:

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. ApoDIRECT In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-12-dUTP at the free 3'-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be observed by fluorescence microscopy or analyzed by flow cytometry.

Application:

Flow Cytometry, Microscopy (fluorescent)

Shelf Life:

12 months

Advantages:

1. One-step labeling of apoptotic cells. 2. Sensitive and stable. 3. Simple, convenient procedure. 4. Takes 1 hour.

Assay Type:

TUNEL Assay

Detection Method:

Fluorescence Microscopy, Flo Cytometry

Kit Contents:

1. Positive Control Cells. 2. Negative Control Cells. 3. Wash Buffer. 4. Reaction Buffer. 5. TdT Enzymes. 6. FITC-dUTP. 7. Rinse Buffer. 8. PI/RNase Staining Buffer.

Other Instruments Required:

Flow cytometer, Fluorescence microscope

Storage Conditions:

Shipped +4°C Ice Packs, Components stored at varying temperatures. See recommended storage details in product insert.

Tags:

Apoptosis

Target:

Apoptosis

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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