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ApoBrdU-IHC DNA Fragmentation Assay Kit

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Description:

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. Apo-BrdU-IHC Assay Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl transferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled anti-BrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H₂O₂/Urea tablets for color generation and methyl green solution for counter staining the cells.

Shelf Life:

12 months

Advantages:

1. One-step labeling of apoptotic cells. 2. Sensitive and stable. 3. Simple, convenient procedure.

Assay Type:

TUNEL Assay

Kit Contents:

1. Control Slides-pos/neg. 2. Blocking Buffer. 3. H₂O₂/Urea Tablets. 4. Protease K. 5. DAB Tablets. 6. TdT Enzymes. 7. Br-dUTP. 8. 200X Conjugate. 9. 5X Reaction Buffer. 10. Anti-BrdU-Biotin Antibody. 11. Methyl Green.

Storage Conditions:

Shipped +4°C Ice Packs, Components stored at varying temperatures. See recommended storage details in product insert.

Tags:

Apoptosis

Target:

Apoptosis

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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