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ApoBrdU DNA Fragmentation Assay Kit (Fluorometric)

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ApoBrdU DNA Fragmentation Assay Kit (Fluorometric)

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Description:

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. Apo-BrdU In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a fluorescein labeled anti-BrdU monoclonal antibody.

Application:

Flow Cytometry, Microscopy (fluorescent)

Shelf Life:

12 months

Advantages:

1. Fast and convenient. 2. Incorporation of BrdU in the procedure provides greater sensitivity than Biotinylated or Digoxigenylated methods. 3. Sensitive and stable. 4. Simple procedure. 5. Takes-1 hour.

Assay Type:

TUNEL Assay

Detection Method:

Fluorometric (Ex/Em 488/520 nm for FITC, and 488/623 nm for PI)

Detection Principle:

TUNEL Assay

Intended Use:

The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections. The incorporation of BrdU in the procedure provides greater sensitivity than biotinylated or digoxigenylated methods.

Kit Contents:

1. Positive Control Cells. 2. Negative Control Cells. 3. Wash Buffer-120 mL. 4. Reaction Buffer. 5. TdT Enzymes. 6. Br-dUTP. 7. Rinse Buffer. 8. Anti-BrdU-FITC Antibody. 9. PI/RNase Staining Buffer.

Other Instruments Required:

Flow cytometer, Fluorescence microscope

Sample:

Cell Cultures, Tissues

Species Reactivity:

Mammal

Storage Conditions:

Shipped -20°C Dry Ice, Components stored at varying temperatures. See recommended storage details in product insert.

Tags:

Apoptosis

Target:

Apoptosis

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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