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Apo-BrdU-Red DNA Fragmentation Assay Kit

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Description:

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. Apo-BrdU-Red In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a red fluorescence labeled anti-BrdU monoclonal antibody. The assay is suitable for studying apoptosis with GFP transfected cells.

Application:

Flow Cytometry

Shelf Life:

12 months

Advantages:

1. Provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections. 2. Sensitive and stable. 3. Simple, convenient procedure. 4. Takes 3 hours.

Assay Type:

TUNEL Assay

Detection Method:

Flo Cytometry (Ex/Em 488/576 nm for BrdU-Red, 488/655 nm for 7-AAD)

Kit Contents:

1. Positive Control Cells. 2. Negative Control Cells. 3. Wash Buffer. 4. Reaction Buffer. 5. TdT Enzymes. 6. Br-dUTP. 7. Rinse Buffer. 8. Anti-BrdU-Red Antibody. 9. 7-AAD/RNase Staining Buffer.

Other Instruments Required:

Flow cytometer

Storage Conditions:

Shipped +4°C Ice Packs, Components stored at varying temperatures. See recommended storage details in product insert.

Tags:

Apoptosis

Target:

Apoptosis

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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