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Annexin V-PE-Cy5 Apoptosis Detection Kit (Fluorometric)

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Annexin V-PE-Cy5 Apoptosis Detection Kit (Fluorometric)

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Description:

The Annexin V-PE-Cy5 Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells, without the need for fixation. Results can be analyzed by flow cytometry or fluorescence microscopy.

Application:

Flow Cytometry, Microscopy (fluorescent)

Shelf Life:

12 months

Advantages:

1. Fast and convenient, no need of fixation of cells. 2. Simple one-step procedure. 3. Takes only 10 minutes.

Assay Type:

Apoptosis Detection

Detection Method:

Fluorometric (Ex/Em 488/670 nm)

Intended Use:

This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Kit Contents:

1. Annexin V-PE-Cy5
2. 1X Binding Buffer
(See Datasheet for specific volumes supplied)

Sample:

Cell Cultures

Species Reactivity:

Mammal

Storage Conditions:

Shipped +4°C Ice Packs, Store at 4°C. Do not freeze.

Tags:

Apoptosis

Target:

Apoptosis

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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