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Annexin V-EGFP Apoptosis Detection Kit (Fluorometric)

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Annexin V-EGFP Apoptosis Detection Kit (Fluorometric)

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Description:

The Annexin V-EGFP Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with an enhanced green fluorescent protein (EGFP) fusion of annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on live cells. Detection can be analyzed by flow cytometry or by fluorescence microscopy with a FITC filter. EGFP is brighter and more photo-stable than other fluorescent reagents. The kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining.

Application:

Flow Cytometry, Microscopy (fluorescent)

Shelf Life:

12 months

Advantages:

1. Differentiates apoptosis vs necrosis when performing both annexin V-EGFP and PI staining EGFP is a bright and photo-stable green fluorescent protein. 2. Fast and convenient. 3. Simple one-step staining procedure. 4. Takes 10 minutes.

Assay Type:

Apoptosis Detection

Detection Method:

Fluorometric (Ex/Em 488/530 nm)

Intended Use:

This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis. EGFP is a bright and photo-stable green fluorescent protein and the kit can differentiate apoptosis vs necrosis when performing both annexin V-EGFP and PI staining.

Kit Contents:

1. Annexin V-EGFP. 2. 1X Binding Buffer. 3. Propidium Iodide (PI).

Other Instruments Required:

Flow cytometer, Fluorescence microscope

Sample:

Cell Cultures

Species Reactivity:

Mammal

Storage Conditions:

Shipped +4°C Ice Packs, Store at 4°C. Do not freeze.

Tags:

Apoptosis

Target:

Apoptosis

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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