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Annexin V-Biotin Apoptosis Detection Kit (Fluorometric)

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Annexin V-Biotin Apoptosis Detection Kit (Fluorometric)

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Description:

The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidin- or avidin-dye reagents, such as(strept)avidin-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -beta-gal, etc.

Application:

Flow Cytometry, Microscopy (fluorescent)

Shelf Life:

12 months

Advantages:

1. Fast and convenient. 2. Simple one step staining procedure. 3. Takes 30 minutes. 4. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any Streptavidine- or Avidin-dye reagents, such as (Strept)Avidine-Fluorescein, -Peroxidase, -Alkaline Phosphatase (AP), and -b-gal, etc.

Assay Type:

Apoptosis Detection

Detection Method:

Fluorometric (Ex/Em 488/530 nm)

Intended Use:

This assay provides for the detection of early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Kit Contents:

1. Annexin V-Biotin. 2. 1X Binding Buffer. 3. Propidium Iodide (PI).

Other Instruments Required:

Flow cytometer, Fluorescence microscope

Sample:

Cell Cultures

Species Reactivity:

Mammal

Storage Conditions:

Shipped +4°C Ice Packs, Store at 4°C. Do not freeze.

Tags:

Apoptosis

Target:

Apoptosis

It should be noted that our products are for research purposes only. Not suitable for any clinical use.

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